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What is blast?

BLAST finds regions of similarity between biological sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance. Learn more The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences.

What is Primer-BLAST?

Ye J, Coulouris G, Zaretskaya I, Cutcutache I, Rozen S, & Madden TL. (2012) "Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction." BMC Bioinformatics 13:134. PubMed

How to use a BLAST search with a subject sequence?

Subject sequence (s) to be used for a BLAST search should be pasted in the text area. It automatically determines the format or the input. To allow this feature there are certain conventions required with regard to the input of identifiers. more... Enter coordinates for a subrange of the subject sequence.

What is the difference between BLASTp and PSI-BLAST?

BlastP simply compares a protein query to a protein database. PSI-BLAST allows the user to build a PSSM (position-specific scoring matrix) using the results of the first BlastP run.

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